You guys are making this specific question too difficult. Wayne12 want to boot a single rom so that it looks like the machine is the original game. He will have nothing other than that one game on there. SO, there is an easy answer that will work and another way that's more complicated depending on what hardware you go with.
Easy way: If you use a joystick/button interface that works in DOS (keyboard encoders like IPAC and KeyWiz) and your video card has DOS supported drivers, you are golden. Set up DOS as normal and then add the correct MAME command line to your autoexec.bat.
Harder way: If you use an interface that requires drivers that are not available for DOS (USB devices like U360 or GP-Wiz for example), then you need to use a different OS. You can go with Windows (WinXP is a very popular choice, but Win98SE or newer will work) or Linux. If you go with Windows, there are guides to hide your OS almost completely both here and other forums around the web. If you use Vista or older, check out InstantSheller. It's an app that will do everything that you need. If you go Linux, I'm sure you can get it working, but I have no advice since I've never set up a distro that wasn't stock.
Good luck and welcome!