When HD's get hot, the metal expands. When metal expands, the drive heads get out of alignment and can do permanent damage to the disk surface and to the read heads.
You definitely will need at least 3/4" between them. Id go with 1" personally.
You will want to use metal - esp aluminum for the sides if possible. Plastic is an awesome heat isulator. (try wearing a 100% acrylic sweater to find out)
You should make half of that storage, and half that backup. Cause if any one of those drives fail.. you will most likely cry.
Im running 4 drives in my system. 2 for storage, 2 for backup. Ive lost at least 4 drives in my short life with pcs. Ive since learned the hard way... that backups are needed. (lost all my data a few times! ):
Ive learned that heat does in fact destroy them quickly. I bought a huge tower case, spaced the drives one full drivespace appart, and put fans in front of them all to keep them very cool. After that, Ive never had a failure anymore, and its been years.
The other thing.. is room tempature. On really hot days, I noticed that the drives were cooking even with the fans. In that case, I removed the towers side door, and placed a huge 2ft box fan right next to the case. And if that didnt work, I turned the pc off. (but that always seemed to do the trick)
If case of an enclosed space such as a cab, you may need to get an industrial blower fan. They move air much quicker than typical case fans. And or use industrial printer fans (or real arcade cab fans). They are almost 1" in thickness, and have heavy duty, powerfull, high rev, ball bearing motors in them. They are rated to move much more air than pc case fans.
I will stress this once more...
- Heat will Destroy HD's in a very short time period.
- Have equal backup's to data (else you are gambling your data, and may lose
huge. HDs have a huge failure rate. I used to work in a pc shop, and I had like 30 bad hds a week to send back cause of failures within less than 1yr service.
Id tried to backup with cds.. and you could try dvd.. but thats actually more costly and time consuming. You will find out that you will forget what you have and have not backed up.. and how little you actually do back up. And if a cd gets a scratch, you will lose that too.
Hd's are great for backup, cause they are so fast to backup, and have such huge space capacity. But backup you must, unless you care less about your data.
Its almost a gaurentee that one day, your drive(s) will fail. They are mechanical in nature, and in time parts will wear and misalign.