I'm well past the 100 hour mark now. I've got a lvl 50 Commando and a lvl 46 Mechromancer. Playthrough 2 difficulty spikes are a bit wonky at times, but it's been an absolute blast. I've played 90% of my time with it as a team of 3 and it's been great. I want to get my mechro up to 50 so I can swap her into my main game (with my friends) some times.
The DLC has been really fun so far as well, and the added raid bosses are a great inclusion. My group finished the first DLC and had a lot of fun with it, and we're about half way through the 2nd DLC. So far, I'm liking that quite a bit as well.
I'm annoyed with some updates though specifically changing the vault hunter (who gives you weapons) appearance rate to 0 and nerfing The Bee (amp shot shield) which may or may not affect the 360 version.
The Mike Mamaril fix was necessary. He'd be in Sanctuary EVERY TIME. That was clearly broken. They also didn't make his appearance rate 0 -- I've seen him 3 or 4 times since the update, if not more.
The Bee nerf sort of needs to happen. the Bee/Conference Call combo is absurdly overpowered. I assume it hasn't been rolled out to 360 yet (where I play) b/c it's still crazy powerful.