For grins, check that your iso transformer is actually isolating. You should not be able to meter any continuity between an input terminal (either one) to an output terminal (either one). If there's a connection there, the transformer has a short in it. Never know when you'll get something DOA.
It doesn't matter which wire is hot/neutral on the iso. In fact, it barely even matters which winding you use for the input and which one you use for the output, but the way you have it hooked up is normally what's the "correct" way.
You might also check that the fuse is fine if you power it up without the monitor attached at all, just the iso trans with no load.
If all that works, you've probably got a problem with the monitor. Various faults will cause it to blow fuses. Since you're getting HV, the horizontal section is probably fine, so check the vertical stuff. The vertical output ICs like to fail shorted, which will blow the fuse.