I don't use plus, but it's probably the same reason as official mame.
Mame now treats each mouse seperately, just like joysticks. If you have two joysticks plugged in, one controls player 1 the other player 2 (by default). Same with mice: you have three, one controls player 1, another player 2, and the third player 3. To get mouse 2 (or joy 2) to control player 1, you need to remap.
If you just want to test the hardware, you might want to just use a 3 player trackball or dial game (rampart or super sprint, respectively). Or just unplug the other two before starting mame.